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Related Experiment Videos

Sequence homologies between type 1 and type 2A protein phosphatases.

G L Johnson1, D L Brautigan, C Shriner

  • 1Department of Biochemistry, University of Massachusetts Medical Center, Worcester 01655.

Molecular Endocrinology (Baltimore, Md.)
|October 1, 1987
PubMed
Summary

Rabbit skeletal muscle type 1 protein phosphatase shares structural similarities with type 2A phosphatase. This suggests protein phosphatases 1 and 2A are closely related gene products within a larger family.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Protein phosphatases are crucial enzymes regulating cellular processes.
  • Type 1 and type 2A protein phosphatases exhibit distinct substrate specificities and inhibitor sensitivities.
  • Understanding the structural relationships between these phosphatases can elucidate their functional divergence.

Purpose of the Study:

  • To investigate the structural homology between type 1 and type 2A protein phosphatases.
  • To provide direct sequence evidence for the evolutionary relationship between these enzyme classes.

Main Methods:

  • Purification and enzymatic digestion of rabbit skeletal muscle type 1 protein phosphatase.
  • Peptide isolation and automated Edman degradation for sequence analysis.

Related Experiment Videos

  • Comparison of sequenced peptides with the deduced sequence of bovine type 2A protein phosphatase cDNA.

    • Main Results:

    • Ten tryptic peptides from type 1 protein phosphatase were sequenced.
    • Nine out of ten sequenced peptides exhibited significant sequence homology with type 2A protein phosphatase.
    • This represents the first direct sequence comparison between type 1 and type 2A protein phosphatases.

    Conclusions:

    • Type 1 and type 2A protein phosphatases share a high degree of structural similarity.
    • These findings support the hypothesis that type 1 and type 2A protein phosphatases are part of a gene family.
    • The conserved structure suggests a common evolutionary origin for these functionally distinct enzymes.