Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

6.0K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
6.0K
Fluid Mosaic Model01:19

Fluid Mosaic Model

18.6K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
18.6K
Electrochemical Gradient and Channel Proteins: An Overview01:21

Electrochemical Gradient and Channel Proteins: An Overview

4.9K
An electrochemical gradient is a fundamental concept in biology and chemistry. It regulates the movement of ions across cell membranes. This movement is influenced by two factors:
The electrical gradient: The electrical gradient across cell membranes refers to the difference in electric charge between the inside and outside of a cell.  This difference drives the movement of ions towards or away from the cells. For instance, if the inside of the cell is more negatively charged relative to...
4.9K
Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

6.7K
In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as...
6.7K
Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

82.4K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
82.4K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

7.7K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Construction of a curated human pharmacokinetics database for molecular fragment analysis and machine learning applications.

Drug metabolism and disposition: the biological fate of chemicals·2026
Same author

Formononetin: a promising therapeutic agent targeting the gut-lung axis in acute lung injury.

Frontiers in pharmacology·2026
Same author

Targeted delivery and ROS-responsive release of celastrol by a macrophage membrane biomimetic liposome alleviates acute kidney injury.

Nanoscale·2026
Same author

Examination of the Utility of Ultrasound in Prenatal Diagnosis and Perinatal Outcomes for 13 Instances of Mirror Syndrome.

Journal of visualized experiments : JoVE·2026
Same author

Clinical and ultrasound-based optimization of post-FNA management decisions in Bethesda III/IV thyroid nodules: a retrospective study.

Frontiers in endocrinology·2026
Same author

Primary intracranial RAF1-rearranged spindle cell neoplasm: a case report of a rare entity.

Acta neurologica Belgica·2026

Related Experiment Video

Updated: Mar 1, 2026

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

3.3K

A Continuum Poisson-Boltzmann Model for Membrane Channel Proteins.

Li Xiao, Jianxiong Diao, D'Artagnan Greene

  • 1Department of Pharmaceutical Sciences, University of Pittsburgh , Pittsburgh, Pennsylvania 15261, United States.

Journal of Chemical Theory and Computation
|June 1, 2017
PubMed
Summary

We developed a new continuum model for membrane protein simulations. This model accurately calculates electrostatic interactions in membrane channels, improving upon existing methods and aligning well with experimental data.

More Related Videos

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
07:47

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

Published on: April 20, 2015

10.4K
Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
07:18

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Published on: January 16, 2019

10.2K

Related Experiment Videos

Last Updated: Mar 1, 2026

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

3.3K
A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
07:47

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

Published on: April 20, 2015

10.4K
Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
07:18

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Published on: January 16, 2019

10.2K

Area of Science:

  • Computational biology
  • Structural biology
  • Biophysics

Background:

  • Membrane proteins are crucial for cellular functions.
  • Computational modeling of membrane proteins is challenging due to their complex environment.
  • Existing continuum models have limitations in accurately representing membrane protein structures.

Purpose of the Study:

  • To introduce a novel continuum model for Poisson-Boltzmann calculations of membrane channel proteins.
  • To enhance the accuracy of computational studies on membrane proteins.
  • To provide a more reliable method for predicting protein-ligand interactions in membrane environments.

Main Methods:

  • Developed a refined continuum slab model incorporating explicit-solvent molecular dynamics (MD) simulations.
  • Implemented a two-step, two-probe grid-labeling procedure to differentiate membrane and water regions.
  • Automated identification of water pores/channels within membrane proteins.
  • Optimized model parameters including slab thickness, center, and membrane probe.
  • Validated the model using binding affinity calculations for a potassium channel.

Main Results:

  • The new model accurately reproduces water molecule distributions in membrane proteins compared to explicit simulations.
  • Optimization confirmed that a 1.4 Å water probe is suitable for membrane protein simulations.
  • Binding affinity calculations for a potassium channel showed good agreement with experimental results.
  • The model effectively addresses differing accessibilities in membrane and water environments.

Conclusions:

  • The proposed continuum membrane model offers significant improvements over existing methods.
  • This model enhances the accuracy of electrostatic calculations for membrane channel proteins.
  • The findings support the use of this model for reliable computational studies and drug discovery involving membrane proteins.