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Polyurethane Microgel Based Microtissue: Interface-Guided Assembly and Spreading.

Michael J Hill1, Debanjan Sarkar1,2

  • 1Department of Biomedical Engineering, University at Buffalo, The State University of New York , Buffalo, New York 14260, United States.

Langmuir : the ACS Journal of Surfaces and Colloids
|June 1, 2017
PubMed
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Researchers engineered three-dimensional microtissues using polyurethane microgels and cells. Microgel stability in aqueous medium influenced cell differentiation, impacting endothelial cell spreading and breast cancer cell lumen formation.

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Colloid Science

Background:

  • Colloidal gels form three-dimensional networks applicable to microtissue engineering.
  • Differential adhesive interactions between particles and cells guide spatial assembly.
  • Surface energetics play a crucial role in designing microtissues.

Purpose of the Study:

  • To design cell-polyurethane (PU) microgel bimodal microtissues using endothelial cells (ECs) and MCF-7 cancer cells.
  • To modulate cell-microgel interactions by engineering PU microgel surface energetics.
  • To investigate the influence of microgel stability on cell behavior and microtissue formation.

Main Methods:

  • Developed PU microgels with tunable surface properties.
  • Estimated surface energies of microgels and cells using contact angle analysis (Zisman, vOGCT).

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  • Calculated interaction potentials (DLVO, XDLVO) and performed rheological analysis and in silico simulations.

    • Main Results:

    • Microgel surface energy modulated interactions with ECs and MCF-7 cells.
    • Stable microgels promoted differentiated cell behavior (EC spreading, MCF-7 lumen formation).
    • Unstable microgels led to undifferentiated cell behavior (EC nonspreading, MCF-7 spreading).

    Conclusions:

    • Colloidal interaction engineering is a viable strategy for designing functional microtissues.
    • Microgel stability is a critical factor controlling cell differentiation within engineered microtissues.
    • This approach offers potential for developing advanced cell-based therapies and tissue models.