Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Classification of the isolated hepatocytes].

S Nagamori1, K Fujise, S Hasumura

  • 1First Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.

Human Cell
|June 1, 1988
PubMed
Summary

This study presents a new method for classifying freshly isolated rat liver cells. The technique separates parenchymal and non-parenchymal cells, enabling detailed functional and morphological analysis for research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of ultrasonographic joint and tendon findings in hands between early, treatment-naïve patients with systemic lupus erythematosus and rheumatoid arthritis.

Lupus·2016
Same author

Influence of Access, Anticoagulant, and Bleeding Definition on Outcomes of Primary Percutaneous Coronary Intervention: Early Experience of an US Academic Center.

The International journal of angiology : official publication of the International College of Angiology, Inc·2015
Same author

Expression of sex-hormone receptors and clinicopathological findings in hepatocellular-carcinoma.

International journal of oncology·2011
Same author

Discontinuation of infliximab after attaining low disease activity in patients with rheumatoid arthritis: RRR (remission induction by Remicade in RA) study.

Annals of the rheumatic diseases·2010
Same author

Efficacy and safety of cyclosporine A in patients with refractory systemic lupus erythematosus in a daily clinical practice.

Lupus·2009
Same author

Severe acute thrombotic exacerbation in two cases with anti-phospholipid syndrome after retreatment with rituximab in phase I/II clinical trial for refractory systemic lupus erythematosus.

Rheumatology (Oxford, England)·2008

Area of Science:

  • Hepatocyte isolation and cell biology
  • Liver research and experimental pathology
  • Biochemical and morphological cell analysis

Context:

  • Enzyme perfusion is standard for isolating rat liver cells for experiments.
  • Accurate classification of isolated liver cells is crucial for reliable experimental outcomes.
  • Existing methods require refinement for precise cell subfractionation.

Purpose:

  • To describe a novel classification method for freshly isolated rat liver cells.
  • To differentiate parenchymal cells into periportal and centrilobular subtypes.
  • To fractionate and characterize non-parenchymal cells, including Kupffer cells and fat-storing cells.

Summary:

  • Hepatocytes were separated from non-parenchymal cells via centrifugation.

Related Experiment Videos

  • A Percoll density gradient further subfractionated parenchymal cells by area.
  • Non-parenchymal cells were classified using vitamin A fluorescence and cytoskeleton morphology.
  • Autoradiography confirmed vitamin A transport into fat-storing cells.

    • Impact:

    • Provides a refined method for liver cell classification.
    • Enables more precise functional and morphological studies of specific liver cell populations.
    • Facilitates deeper understanding of liver physiology and pathology through improved cell analysis.