Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential generation of parasite-specific Th2 and T follicular helper cells distinguishes resistant and susceptible mouse strains.

Frontiers in immunology·2026
Same author

Impact of a Dietary Fish Oil Supplementation on the Plasma Lipidome of Healthy Adult Cats.

Metabolites·2026
Same author

Protective efficacy of a single-domain ASP-based vaccine against the bovine intestinal nematode Cooperia oncophora.

Scientific reports·2026
Same author

Development and preliminary evaluation of two novel PCR assays for improved detection of <i>Taenia saginata</i> and <i>Taenia solium</i> eggs.

Food and waterborne parasitology·2026
Same author

Bistort rhizome extract enhances intestinal barrier integrity in a porcine intestinal epithelial cell model.

Fitoterapia·2026
Same author

Toward reproducible pig gut microbiome profiling through standardized methodologies.

ISME communications·2026

Related Experiment Video

Updated: Mar 1, 2026

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
08:13

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Published on: January 20, 2019

22.6K

Pathogen-Reactive T Helper Cell Analysis in the Pig.

Friederike Ebner1, Patrycja Schwiertz1, Svenja Steinfelder1

  • 1Department of Veterinary Medicine, Institute of Immunology, Freie Universität Berlin, Berlin, Germany.

Frontiers in Immunology
|June 2, 2017
PubMed
Summary

Researchers identified CD154 (CD40L) as a marker for porcine antigen-reactive T cells. This discovery aids in studying host-pathogen interactions and immune responses in pigs.

Keywords:
Ascaris suumCD154CD40 ligandCandida albicansStreptococcus suisantigen-specificpigporcine CD4 T cell

More Related Videos

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches
08:25

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Published on: November 21, 2018

14.3K
Application of Long-term cultured Interferon-&#947; Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
15:57

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Published on: July 11, 2015

12.8K

Related Experiment Videos

Last Updated: Mar 1, 2026

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
08:13

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Published on: January 20, 2019

22.6K
Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches
08:25

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Published on: November 21, 2018

14.3K
Application of Long-term cultured Interferon-&#947; Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
15:57

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Published on: July 11, 2015

12.8K

Area of Science:

  • Immunology
  • Veterinary Science
  • Host-Pathogen Interactions

Background:

  • Studying host-pathogen interactions in large animal models like pigs is crucial.
  • Assessing pathogen-specific T cells, vital for immune response, remains challenging in pigs due to their rarity.

Purpose of the Study:

  • To establish CD154 (CD40L) as a reliable marker for identifying porcine antigen-reactive CD4+ T lymphocytes.
  • To enable detailed analysis of T cell responses in pigs against various pathogens.

Main Methods:

  • Utilized CD154 (CD40L) as an activation marker for porcine T cells.
  • Employed antigen-induced expansion and autologous restimulation for detailed analysis.
  • Phenotypically profiled antigen-responsive cells using CD154 expression.

Main Results:

  • CD154 expression successfully identified porcine CD4+ T lymphocytes reactive to specific antigens.
  • Upregulation of CD154 was observed early after antigen encounter, with coexpression of cytokines.
  • Detected antigen-reactive T cells against *Staphylococcus aureus* superantigen, *Candida albicans*, *Ascaris suum*, and *Streptococcus suis*.

Conclusions:

  • CD154 is a valuable marker for identifying and studying antigen-specific T cells in pigs.
  • This method enhances the understanding of porcine immune responses in host-pathogen interactions.
  • Provides novel tools for investigating T cell immunity in a key animal model.