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Updated: Mar 1, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
Purification of Plant Receptor Kinases from Plant Plasma Membranes
1Department of Biology, Concordia University, 7141 Sherbrooke W., Montreal, QC, Canada, H4B 1R6. jinsuk.lee@concordia.ca.
Abstract:
Receptor kinases play a central role in various biological processes, but due to their low abundance and highly hydrophobic and dynamic nature, only a few of them have been functionally characterized, and their partners and ligands remain unidentified. Receptor protein extraction and purification from plant tissues is one of the most challenging steps for the success of various biochemical analyses to characterize their function. Immunoprecipitation is a widely used and selective method for enriching or purifying a specific protein. Here we describe two different optimized protein purification protocols, batch and on-chip immunoprecipitation, which efficiently isolate plant membrane receptor kinases for functional analysis.
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