Fission yeast Ctf1, a cleavage and polyadenylation factor subunit is required for the maintenance of genomic integrity
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Updated: Mar 1, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
Published on: June 24, 2019
Amit Sonkar1,2, Sachin Gaurav1, Shakil Ahmed3
1Molecular and Structural Biology Division, CSIR, Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, 226031, India.
Abstract:
Accurate segregation of chromosome during mitosis requires the coordinated action of several cell cycle checkpoints that monitor replication of the genome and the attachment of sister chromatids to the mitotic spindle apparatus. Here we have characterized the fission yeast Ctf1, an ortholog of S. cerevisiae Rna15 in the maintenance of genomic integrity. The ctf1 is nonessential for the cell survival and its deletion strain exhibit cold sensitivity. The ctf1 deleted cells exhibit genetic interaction with spindle checkpoint protein Mad2 and Bub1. The deletion of ctf1 gene affects the chromosomal attachment to the mitotic spindle leading to the accumulation of Bub1-GFP foci. Ctf1 localizes to the nucleus and physically interacts with Rna14, a cleavage and polyadenylation factor.
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