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Complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a
M I Darville1, K M Crepin, J Vandekerckhove
1Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Gent, Belgium.
FEBS Letters
|November 30, 1987
Summary
Researchers isolated cDNA clones for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from rat liver. The full-length nucleotide sequence for this bifunctional enzyme
Area of Science:
- Biochemistry
- Molecular Biology
- Enzymology
Background:
- 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is a key bifunctional enzyme regulating glycolysis.
- Understanding its structure is crucial for metabolic pathway research.
Purpose of the Study:
- To isolate and characterize cDNA clones encoding rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
- To determine the full nucleotide sequence and subunit structure of the enzyme.
Main Methods:
- Screening of rat liver expression libraries in lambda gt11 using antibody, oligonucleotide, and cDNA probes.
- Nucleotide sequencing of isolated cDNA clones.
Main Results:
- Isolation of an 1860 bp cDNA clone containing the full-length coding sequence.
- The clone encodes a 470 amino acid protein for each of the two identical subunits.
- The clone includes upstream (173 bp) and downstream (271 bp) untranslated regions and a poly(A) tail.
Conclusions:
- The isolated cDNA clone provides the complete nucleotide sequence for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
- This sequence information is vital for further studies on enzyme function and regulation.