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Related Experiment Video

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Cell sheet based bioink for 3D bioprinting applications.

E Bakirci1, B Toprakhisar, M C Zeybek

  • 1Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey. 3D Bioprinting Lab, Sabanci University Nanotechnology Research and Application Center, Istanbul, Turkey.

Biofabrication
|June 2, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel bioink from cell sheets for scaffold-free bioprinting. This cell sheet bioink enhances structural integrity and preserves cell-matrix interactions, offering advantages for tissue engineering applications.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Regenerative Medicine

Background:

  • Scaffold-free bioprinting aims to create 3D tissue constructs by preserving cell-cell and cell-extracellular matrix (ECM) interactions.
  • Existing bioinks often struggle to maintain structural integrity and native cell-ECM connections during printing.

Purpose of the Study:

  • To develop a novel bioink derived from cell sheets for scaffold-free bioprinting applications.
  • To evaluate the structural integrity, cell viability, and ECM deposition of the developed cell-sheet-based bioink.

Main Methods:

  • Preparation of thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) coated surfaces using initiated chemical vapor deposition.
  • Culturing cell sheets on PNIPAAm surfaces, detaching them non-disruptively, and processing into a cell-sheet-based bioink.
  • Bioprinting the cell-sheet bioink into various shapes and assessing structural integrity, cell viability, and ECM deposition (collagen type I, fibronectin).

Main Results:

  • The cell-sheet-based bioink exhibited enhanced structural integrity compared to single cell aggregates, preserving interconnected ECM proteins.
  • High cell viability was maintained in cell-sheet aggregates over a seven-day period post-printing.
  • Bioprinted constructs demonstrated secretion of collagen type I and preservation of fibronectin, indicating ECM deposition and maintenance.

Conclusions:

  • A novel and advantageous cell-sheet-based bioink for scaffold-free bioprinting has been successfully developed.
  • This bioink offers improved shape fidelity, reproducibility, and automated deposition capabilities.
  • The findings suggest potential for creating functional tissue constructs with preserved cellular microenvironments.