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Related Experiment Videos

Phenotypic variation in clonal Abelson virus lymphoma cells.

P L Green, W W Lamph, J Dudley

    Journal of Immunology (Baltimore, Md. : 1950)
    |February 1, 1985
    PubMed
    Summary

    This study shows that lymphoma cell lines can develop new types of cells with different markers when grown in vivo. These new cell variants can become the main type of tumor cell over time.

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    Area of Science:

    • Immunology
    • Oncology
    • Cell Biology

    Background:

    • Murine leukemia viruses (MuLV) can induce lymphomas.
    • Lymphoma cell lines can exhibit phenotypic plasticity.
    • Understanding neoplastic progression requires studying cellular differentiation markers.

    Purpose of the Study:

    • To investigate the generation and characteristics of phenotypic variants from A-MuLV lymphoma cell lines in vivo.
    • To model neoplastic differentiation and progression in the lymphoid system.

    Main Methods:

    • In vivo growth of A-MuLV lymphoma cell lines as ascites tumors.
    • Analysis of cell surface marker expression (B220, Lyb-2, Thy-1, Lyt-1) using monoclonal antibodies.
    • Assessment of enzymatic activity (TdT).

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  • Cell cloning and quantification of variant populations.

    • Main Results:

    • Parental lymphoma cells expressed pre-B cell markers (B220, Lyb-2) and low TdT activity.
    • Three classes of phenotypic variants were identified based on marker expression (Thy-1, Lyt-1, 14.8).
    • Thy-1+ variants emerged from Thy-1- parental lines within 14 days and became predominant.

    Conclusions:

    • Phenotypic variants arise from A-MuLV lymphoma cells during in vivo growth.
    • These variants exhibit altered differentiation marker expression.
    • The generated clonal tumor lines serve as a valuable model for studying lymphoid neoplastic progression.