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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
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A colorimetric and fluorogenic probe for bisulfite using benzopyrylium as the recognition unit.

Yun Zhang1,2, Xiangwen Zhang1, Xiao-Feng Yang1

  • 1Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, People's Republic of China.

Luminescence : the Journal of Biological and Chemical Luminescence
|June 2, 2017
PubMed
Summary

A new coumarin-benzopyrylium (CB) probe enables sensitive and selective detection of bisulfite. This fluorescent method offers rapid results for bisulfite analysis in various samples.

Keywords:
benzopyryliumbisulfitecoumarinfluorescent probe

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Area of Science:

  • Analytical Chemistry
  • Organic Chemistry
  • Biochemistry

Background:

  • Bisulfite is a crucial analyte in various chemical and biological processes.
  • Accurate detection of bisulfite is essential for quality control and biological studies.
  • Existing detection methods may lack sensitivity, selectivity, or speed.

Purpose of the Study:

  • To develop a novel fluorescent probe for sensitive and selective bisulfite detection.
  • To investigate the mechanism of probe-target interaction.
  • To evaluate the probe's performance in real samples and biological systems.

Main Methods:

  • Synthesis of a coumarin-benzopyrylium (CB) probe.
  • Utilizing nucleophilic addition of bisulfite to the benzopyrylium moiety.
  • Monitoring changes in fluorescence emission intensity at 510 nm.
  • Assessing probe response time, selectivity, and sensitivity.

Main Results:

  • The CB probe demonstrated a linear response to bisulfite in the range of 0.2-7.5 μM.
  • A low detection limit of 42 nM was achieved.
  • The probe exhibited rapid response (within 30 s), high selectivity, and sensitivity.
  • Successful preliminary detection of bisulfite in real samples and living cells.

Conclusions:

  • The CB platform provides an effective tool for colorimetric and fluorogenic bisulfite detection.
  • The probe's mechanism involves the inactivation of the oxonium ion, enhancing coumarin fluorescence.
  • CB shows significant potential for practical applications in bisulfite analysis.