Evaluating the Procedure for Performing Awake Cystometry in a Mouse Model.

Area of Science:

• Urology
• Physiology
• Surgical techniques

Background:

• Awake cystometry is crucial for evaluating bladder function in freely moving mice.
• Existing methods for awake cystometry vary significantly between laboratories.
• Standardized techniques are needed for reliable intravesical pressure and voided volume recording.

Purpose of the Study:

• To describe a microsurgical procedure for implanting an intravesical tube in mice.
• To detail the experimental technique for recording urinary bladder pressure in awake, freely moving mice.
• To present data on how surgical factors and tubing characteristics influence lower urinary tract function and recording sensitivity.

Main Methods:

• Microsurgical implantation of intravesical tubes (polyethylene and polyurethane) into the bladder dome of C57BL/6 mice.
• Assessment of tube diameter effects on pressure recordings.
• Recording of 12-hour overnight micturition frequency in healthy and post-surgery mice (days 2, 3, 5, 7).
• Gross observation and pathological analysis of bladder tissue for swelling and inflammation.

Main Results:

• Bladder swelling and impaired voiding function were most significant on post-operative days 2 and 3.
• Histological analysis confirmed swelling, which resolved by post-operative day 5.
• Voiding frequency normalized by post-operative day 5, indicating functional recovery.
• Polyethylene-10 tubing was identified as optimal for minimizing movement artifacts.

Conclusions:

• A standardized microsurgical technique for awake cystometry in mice has been established.
• Optimal timing for cystometry is post-operative day 5 to ensure bladder recovery and accurate readings.
• The use of polyethylene-10 tubing is recommended for improved recording sensitivity and reduced artifacts in awake mouse cystometry.