Related Experiment Video
Updated: Mar 1, 2026

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
Published on: December 16, 2015
Lipid profile of different infant formulas for infants
Marcio Antonio Mendonça1, Wilma Maria Coelho Araújo2, Luiz Antonio Borgo1
1Faculty of Agronomy and Veterinary Medicine, University of Brasilia, Brasilia, Brazil.
Insights
Infant formulas differ significantly from human milk in lipid content and fatty acid profiles. However, formulas like Soy Protein Isolate-based Infant Formula (SPIIF) and Whey Protein Extensively Hydrolyzed Infant Formula (WPEHIF) show similarities, making them viable alternatives.
Area of Science:
- Nutritional Science
- Pediatric Nutrition
- Food Chemistry
Background:
- Infant formulas are essential when breastfeeding is not possible, especially for premature infants.
- Human milk is the gold standard for infant nutrition.
- Understanding the lipid composition of infant formulas is crucial for infant development.
Purpose of the Study:
- To determine the lipid content of various infant formulas.
- To identify the specific lipid profiles of these formulas.
- To compare the lipid composition of infant formulas with that of human milk.
Main Methods:
- Analysis of lipid content and fatty acid profiles in ten different infant formulas and pooled human milk.
- Utilized a randomized block design with eleven treatments and three replicates.
- Statistical analysis to identify significant differences.
Main Results:
- Significant differences were observed in total lipid percentage and fatty acid profiles between infant formulas and human milk.
- Exceptions included linoleic acid and linolenic acid fractions.
- Soy Protein Isolate-based Infant Formula (SPIIF) and Whey Protein Extensively Hydrolyzed Infant Formula (WPEHIF) showed comparable polyunsaturated fatty acid percentages to human milk.
Conclusions:
- Despite compositional differences, infant formulas are a viable nutritional strategy for infants.
- Specific formulas, such as SPIIF and WPEHIF, offer lipid profiles closer to human milk.
- Further research into optimizing infant formula lipid composition is warranted.
Abstract:
Situations including premature infants, or those in which there is a rejection to breastfeeding, require the use infant formulas for total or partial replacement of human milk. The objective of this study was to determine the lipid content and to identify the lipid profile of infant formulas. Samples were collected from ten different infant formulas, used as a substitute for breast milk at the Maternal and Child Hospital of Brasilia. The human milk sample consisted of a pool of samples from 10 mature milk donors at the milk bank of the University Hospital of Brasilia. The lipid content and lipid profile of the different infant formulas and human milk were analyzed. The experiment was conducted in a randomized block design, with eleven treatments and three replicates, in triplicate. The data obtained in this study indicated significant differences between infant formulas and human milk, and among the infant formulas analyzed in relation to the percentage of total lipids and the fatty acid profile, except for the fractions of linoleic acid and linolenic acid. Regarding the percentage of polyunsaturated fatty acids in relation to the total unsaturated fatty acids, only the Soy Protein Isolate-based Infant Formula (SPIIF) and Whey Protein Extensively Hydrolyzed Infant Formula (WPEHIF) resembled human milk. It was concluded that despite the observed differences, the use of infant formulas is a viable strategy for the development of infants subjected or not to specific physiological conditions.
Related Concept Videos
Pharmacokinetics in Pediatric Patients: Drug Distribution
Pharmacokinetics in Pediatric Patients: Overview and Drug Absorption
Blood Studies for Cardiovascular System III: Serum Lipid Profile
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
Lipid Absorption
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
Lipid-derived Compounds in the Human Body
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
Drug Dosing: Infants and Children

