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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
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The Ser/Thr Protein Kinase Protein-Protein Interaction Map of M. tuberculosis

Fan-Lin Wu1,2, Yin Liu1,2, He-Wei Jiang1,2

  • 1From the ‡Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.

Molecular & Cellular Proteomics : MCP
|June 3, 2017
PubMed

Insights

Researchers mapped protein interactions for Mycobacterium tuberculosis serine/threonine protein kinases (STPKs). This reveals key signaling pathways involved in tuberculosis pathogenesis, aiding new drug development against this infectious disease.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Mycobacterium tuberculosis (Mtb) causes tuberculosis, a leading infectious disease mortality.
  • Eleven serine/threonine protein kinases (STPKs) in Mtb are implicated in vital cellular processes but their functions are unclear.

Purpose of the Study:

  • To globally identify binding proteins for all Mtb STPKs.
  • To construct the first comprehensive STPK protein interaction (KPI) map for Mtb.

Main Methods:

  • Utilized a Mtb proteome microarray for global protein interaction identification.
  • Performed bioinformatics analysis on the generated STPK-KPI network.

Main Results:

  • Generated the first Mtb STPK-KPI map with 492 binding proteins and 1,027 interactions.
  • Identified diverse functions of interacting proteins, including roles in two-component systems, transcription, and cell wall integrity.
  • Confirmed PknG regulates cell wall integrity via peptidoglycan biosynthesis components like MurC.

Conclusions:

  • The global STPK-KPI network provides a valuable resource for understanding Mtb signaling pathways.
  • This knowledge can facilitate the development of novel drugs and strategies for tuberculosis control.

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