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Related Concept Videos

DNA Isolation01:34

DNA Isolation

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DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
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DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
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Concentrating Nucleic Acids by Extraction with Butanol.

Michael R Green, Joseph Sambrook

    Cold Spring Harbor Protocols
    |June 3, 2017
    PubMed
    Summary

    Concentrate dilute nucleic acid solutions by extracting water into organic solvents like butanol. Sequential extractions significantly reduce volume, enabling easy recovery via ethanol precipitation.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Organic Chemistry

    Background:

    • Nucleic acid isolation often yields dilute solutions requiring concentration.
    • Traditional concentration methods can be time-consuming or lead to sample loss.

    Purpose of the Study:

    • To present a simple, effective method for concentrating nucleic acid solutions.
    • To demonstrate the utility of organic solvent extraction for nucleic acid sample preparation.

    Main Methods:

    • Utilizing secondary butyl alcohol (isobutanol) or n-butyl alcohol (n-butanol) for aqueous extraction.
    • Performing sequential extraction cycles to remove water into the organic phase.
    • Recovering concentrated nucleic acids via ethanol precipitation.

    Main Results:

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    • Water molecules are preferentially partitioned into the organic solvent phase.
    • Nucleic acids remain in the aqueous phase throughout the extraction process.
    • Sequential extractions achieve significant volume reduction of nucleic acid solutions.

    Conclusions:

    • Organic solvent extraction provides an efficient method for nucleic acid concentration.
    • This technique facilitates the recovery of nucleic acids from dilute aqueous solutions.
    • The method is compatible with subsequent ethanol precipitation for isolation.