Related Experiment Video
Updated: Mar 1, 2026
09:44
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
3.0K
Genome Sequence of Escherichia coli E28, a Multidrug-Resistant Strain Isolated from a Chicken Carcass, and Its
Imke Schmidt1, Thomas Riedel2, Isabel Schober1
1Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Genome Announcements
|June 3, 2017
Abstract:
In this study, we sequenced the complete genome of the multidrug-resistant Escherichia coli strain E28, which was used as an indicator strain for phage therapy in vivo We used a combination of single-molecule real-time and Illumina sequencing technology to reveal the presence of a spontaneously inducible prophage.
Insights
Researchers sequenced the multidrug-resistant Escherichia coli strain E28 genome. This revealed a spontaneously inducible prophage, important for phage therapy research in vivo.
Area of Science:
- Microbiology
- Genomics
- Bacteriophage Research
Background:
- Multidrug-resistant bacteria pose a significant global health threat.
- Phage therapy is an emerging alternative to antibiotics for bacterial infections.
- Characterizing bacterial genomes is crucial for understanding resistance mechanisms and developing targeted therapies.
Purpose of the Study:
- To perform a complete genome sequencing of the multidrug-resistant Escherichia coli strain E28.
- To identify genetic elements relevant to phage therapy applications.
Main Methods:
- Whole-genome sequencing using a combination of single-molecule real-time (SMRT) and Illumina technologies.
- Bioinformatic analysis for genome assembly and feature identification.
Main Results:
- The complete genome sequence of Escherichia coli strain E28 was successfully obtained.
- A spontaneously inducible prophage was identified within the E28 genome.
- Strain E28's genomic data provides a foundation for its use as an indicator strain in phage therapy studies.
Conclusions:
- The genomic characterization of Escherichia coli E28 provides valuable insights into its genetic makeup.
- The identified prophage is a key feature for future investigations in phage therapy efficacy.
- This study supports the use of strain E28 as a model organism for in vivo phage therapy research.
More Related Videos
Related Concept Videos
01:03Single-Strand DNA Binding Proteins
17.0K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
17.0K
00:46Genomic DNA in Prokaryotes
49.4K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
49.4K
00:43Lysogenic Cycle of Bacteriophages
68.3K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
68.3K
01:26DNA Bacteriophages
1.2K
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
1.2K
01:20Viral Replication: Lytic Cycle
2.1K
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
2.1K
01:23Stringent Response in E. coli
420
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
420