Complete Genome Sequences of Two Geographically Distinct Legionella micdadei Clinical Isolates
Amy J Osborne1,2, Bethany R Jose3, Jasper Perry3
1School of Biological Sciences, University of Canterbury, Christchurch, New Zealand amy.osborne@otago.ac.nz sandy.slow@otago.ac.nz.
Abstract:
Legionella is a highly diverse genus of intracellular bacterial pathogens that cause Legionnaire's disease (LD), an often severe form of pneumonia. Two L. micdadei sp. clinical isolates, obtained from patients hospitalized with LD from geographically distinct areas, were sequenced using PacBio SMRT cell technology, identifying incomplete phage regions, which may impact virulence.
Insights
Researchers sequenced two Legionella micdadei isolates from Legionnaire's disease patients. Incomplete phage regions were identified, potentially influencing the bacteria's virulence and disease severity.
Area of Science:
- Microbiology
- Bacterial Pathogenesis
- Genomics
Background:
- Legionella is a genus of intracellular bacteria responsible for Legionnaire's disease (LD), a severe pneumonia.
- Clinical isolates of Legionella species are crucial for understanding disease mechanisms.
Purpose of the Study:
- To sequence and analyze clinical isolates of *L. micdadei* from distinct geographical locations.
- To investigate the presence and potential impact of mobile genetic elements, such as phages, on *L. micdadei* virulence.
Main Methods:
- Whole-genome sequencing of two *L. micdadei* clinical isolates using PacBio SMRT cell technology.
- Bioinformatic analysis to identify genomic features, including phage sequences.
Main Results:
- The genome sequences revealed the presence of incomplete phage regions within both *L. micdadei* isolates.
- These phage regions represent novel genetic elements within these specific clinical strains.
Conclusions:
- The identified incomplete phage regions in *L. micdadei* may play a role in the bacterium's virulence.
- Further research is warranted to elucidate the functional significance of these phage elements in the pathogenesis of Legionnaire's disease.
