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WITHAFERIN A INDUCES APOPTOSIS IN RAT C6 GLIOMA CELLS THROUGH REGULATING NF-KB NUCLEAR TRANSLOCATION AND ACTIVATION

Wei-Chen Hou1, Xiao-Hui Miao2, Lian-Jun Ma3

  • 1Department of Neurology, The First Hospital of Jilin University, Changchun 130021, China.

African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM
|June 3, 2017
PubMed
Abstract

Insights

Withaferin A (WFA) significantly reduces C6 glioma cell proliferation and inflammation while promoting apoptosis. This plant-derived compound shows dose-dependent anti-glioma effects, indicating its potential as a chemopreventive agent.

Area of Science:

  • Natural product chemistry
  • Cancer biology
  • Pharmacology

Background:

  • Increasing demand for plant-derived chemopreventive drugs with minimal adverse effects.
  • Withaferin A (WFA) is a compound with known biological activities.
  • Need to investigate WFA's potential against glioma.

Purpose of the Study:

  • To evaluate the anti-glioma effects of Withaferin A (WFA) on the C6 glioma cell line.
  • To assess WFA's impact on glioma cell proliferation, inflammation, and apoptosis.

Main Methods:

  • C6 glioma cells were treated with varying concentrations of WFA (50-500 μg/mL).
  • Dimethyl sulfoxide (DMSO) was used as the control.
  • Assessed anti-proliferative, anti-inflammatory, and pro-apoptotic activities.

Main Results:

  • WFA demonstrated a dose-dependent reduction in glioma cell count (anti-proliferative effect).
  • Inflammatory markers were significantly decreased.
  • Apoptotic markers (Caspase-3, Caspase-9, DNA fragmentation) increased, indicating cytotoxicity.
  • WFA modulated Bcl2 and Bax protein expression, favoring apoptosis.

Conclusions:

  • Withaferin A exhibits significant anti-proliferative, anti-inflammatory, and pro-apoptotic effects on C6 glioma cells.
  • These effects are dose-dependent.
  • WFA shows promise as a potential therapeutic agent for glioma.
Keywords:
C6 glioma cellsWithaferin Aanti-inflammatoryanti-proliferativeapoptosiscaspase

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