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Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation
Logan Banadyga1, Hideki Ebihara2,3
1Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2017
Abstract:
Immunoprecipitations are commonly used to isolate proteins or protein complexes and assess protein-protein interactions; however, they can also be used to assess protein-RNA complexes. Here we describe an adapted RNA immunoprecipitation technique that permits the quantification of RNA content in Ebola virus nucleocapsids that have been reconstituted in vitro by transient transfection.
