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Updated: Mar 1, 2026
Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells
Published on: July 3, 2017
Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence
Emily V Nelson1, Kristina M Schmidt2,3
1Department of Microbiology and National Emerging Infectious Diseases Laboratories, School of Medicine, Boston University, Boston, MA, USA.
Abstract:
In this chapter, the use of immunofluorescence analysis as a tool to examine stress granule (SG) formation in Ebola virus (EBOV)-infected cells is described. The following protocol focuses on the process of inducing and analyzing the cellular stress response, including treatment of cells with inducers and inhibitors of the SG formation, and also describes EBOV infection, DNA transfection, and the usage of different cell lines.
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