Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells
Judith Olejnik1, Emily V Nelson2
1Department of Microbiology and National Emerging Infectious Diseases Laboratories, School of Medicine, Boston University, Boston, MA, USA. jolejnik@bu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2017
Abstract:
In this chapter, we describe a Western blot assay for the successful detection of apoptosis in Ebola virus (EBOV)-infected cells. The protocol includes all steps from cell culture, infection of cells, generation of lysates, and analysis using Western blot to detect caspase cleavage as marker of apoptosis.
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