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Related Experiment Video

Updated: Mar 1, 2026

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
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Optical clearing of the eye using the See Deep Brain technique.

B Hohberger1, C Baumgart1, A Bergua1

  • 1Department of Ophthalmology and Eye Hospital, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.

Eye (London, England)
|June 3, 2017
PubMed
Summary

The See Deep Brain (SeeDB) method achieved complete scleral transparency in human and chicken eyes. This optical clearing technique enables visualization of intraocular structures without dissection, advancing anatomical studies.

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Related Experiment Videos

Last Updated: Mar 1, 2026

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Area of Science:

  • Ophthalmology
  • Neuroanatomy
  • Histology

Background:

  • Tissue clearing techniques have advanced neuronal tissue visualization.
  • Optical clearing methods like See Deep Brain (SeeDB) have not been applied to ocular tissues.
  • The eye, part of the central nervous system, presents unique challenges for histological analysis.

Purpose of the Study:

  • To apply the See Deep Brain (SeeDB) optical clearing method to visualize intraocular structures.
  • To assess the efficacy of SeeDB in achieving scleral transparency for enhanced anatomical study.
  • To explore the potential for SeeDB in future topographical and pathological studies of the eye.

Main Methods:

  • Human (cornea donor) and animal (chicken, mouse) eyes were fixed.
  • Samples were treated with SeeDB, a water-based optical clearing method using fructose and α-thioglycerol.
  • Transscleral macrophotography of the choroid was performed after clearing.

Main Results:

  • Complete scleral transparency was achieved in human and chicken eyes post-SeeDB treatment.
  • Macroscopical visualization of choroidal vasculature and structures was possible through the translucent sclera.
  • Mice optic nerves also became transparent after SeeDB application.

Conclusions:

  • The SeeDB method successfully renders the sclera transparent, enabling visualization of internal ocular structures.
  • This technique facilitates 3D topographical anatomical studies of human and animal eyes.
  • SeeDB holds potential for visualizing structures like choroidal ganglionic plexuses and aiding clinical-pathological correlations in intact eyes.