Induction of apoptosis in human colorectal cancer cell line, HCT-116 by a vanadium- Schiff base complex
Abhinaba Sinha1, Kaushik Banerjee1, Arpita Banerjee1
1Department of In Vitro Carcinogenesis and Cellular Chemotherapy, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700 026, India.
Abstract:
Vanadium compounds are well known for their therapeutic interventions against several diseases. Various biochemical attributes of vanadium complexes inspired us to evaluate the cancer cell killing efficacy of the vanadium complex, viz., vanadyl N-(2-hydroxyacetophenone) glycinate [VO(NG)2]. Previously we showed that VO(NG)2 is an effective anticancer agent in in vitro and in vivo cancer models and imposed miniscule side effects. Herein we report that VO(NG)2 is significantly cytotoxic to various cancer cell lines. Furthermore, this redox active vanadyl complex altered the redox homeostatsis of many human cancer cell lines significantly. VO(NG)2 actuates programmed cell death in human colorectal carcinoma cells(HCT-116) through mitochondrial outer membrane permeabilization but in caspase independent manner, possibly by altering cellular redox status and by inflicting DNA damage. Thus, the present work is an attempt to provide many evidences regarding the potent and selective chemotherapeutic efficacy of the novel VO(NG)2.
Insights
The novel vanadium complex, vanadyl N-(2-hydroxyacetophenone) glycinate [VO(NG)2], demonstrates significant cancer cell killing efficacy. This redox-active compound induces programmed cell death in human colorectal carcinoma cells via a caspase-independent pathway.
Area of Science:
- Medicinal Chemistry
- Biochemistry
- Cancer Biology
Background:
- Vanadium compounds are recognized for their therapeutic potential across various diseases.
- The biochemical properties of vanadium complexes prompted an investigation into their anticancer capabilities.
- Previous studies established vanadyl N-(2-hydroxyacetophenone) glycinate [VO(NG)2] as an effective anticancer agent with minimal side effects.
Purpose of the Study:
- To evaluate the cytotoxic effects of vanadyl N-(2-hydroxyacetophenone) glycinate [VO(NG)2] on diverse cancer cell lines.
- To investigate the mechanism by which VO(NG)2 induces cell death in human colorectal carcinoma cells (HCT-116).
Main Methods:
- Cytotoxicity assays were performed on various human cancer cell lines treated with VO(NG)2.
- The impact of VO(NG)2 on cellular redox homeostasis was assessed.
- Mechanistic studies involving mitochondrial outer membrane permeabilization and DNA damage were conducted in HCT-116 cells.
Main Results:
- VO(NG)2 exhibited significant cytotoxicity against multiple human cancer cell lines.
- The vanadyl complex markedly disrupted the redox homeostasis in treated cancer cells.
- VO(NG)2 induced caspase-independent programmed cell death in HCT-116 cells, associated with mitochondrial outer membrane permeabilization and DNA damage.
Conclusions:
- VO(NG)2 possesses potent and selective chemotherapeutic efficacy.
- The compound's ability to alter cellular redox status and induce DNA damage contributes to its anticancer activity.
- VO(NG)2 represents a promising novel chemotherapeutic agent for cancer treatment.
