Tailor made alginate hydrogel for local infection prophylaxis in orthopedic applications

Preventing implants associated infections is crucial in orthopedics. Local delivery of antibiotics through implants is a promising strategy to solve this issue. In this study, alginate is tailored to control its swelling, entrapment and release of ciprofloxacin (antibiotic) through the formation of interpenetrating polymer network and composite matrices using gelatin and hydroxyapatite. Developed matrices were characterized by Fourier transform infrared spectroscopy, x-ray diffraction and scanning electron microscopy. The individual tailoring approaches exerted significant influence on the swelling behavior of alginate matrix consequently enhanced the drug entrapment and extended the release period. However, the alginate matrix tailored with the combined approaches resulted in a superior matrix, which had a better control over the burst release of ciprofloxacin. Drug release prolonged from 5h to 240h for composite matrix as compared with alginate matrix showing that alginate combined with gelatin and hydroxyapatite sustained the release for longer periods. This matrix revealed excellent biocompatibility with osteoblast like MG-63 cell lines and showed good antibacterial activity against S. aureus and E. coli.