Insight into cytotoxicity of Mg nanocomposites using MTT assay technique

In the present study, Young's modulus measurements and indirect cytotoxicity test were performed on Mg (0.97, 1.98, 2.5) vol% TiO₂, Mg (0.58, 0.97, 1.98) vol% TiC, and Mg (0.58, 0.97, 1.98) vol% TiN, synthesized using the Disintegrated Melt Deposition technique to determine the cytotoxicity of low volume nano-particulate reinforcement on magnesium. The results of the indirect MTT assay on Day 3 and Day 5 indicate that 2.5vol% TiO₂, TiC and TiN has little effect on the cytotoxicity when added as low volume fraction reinforcement to magnesium. While (0.97, 1.98) vol% TiO₂ negatively affected the cytotoxicity when added to Mg. The Young's modulus of the materials was found to remain close to that of cortical bone which would suggest that the stress shielding effect would be reduced as the increase in Young's modulus was mitigated by the low volume addition of reinforcements.