Distinct physiological and molecular responses in Arabidopsis thaliana exposed to aluminum oxide nanoparticles and...

Nano-aluminium oxide (nAl₂O₃) is one of the most widely used nanomaterials. However, nAl₂O₃ toxicity mechanisms and potential beneficial effects on terrestrial plant physiology remain poorly understood. Such knowledge is essential for the development of robust nAl₂O₃ risk assessment. In this study, we studied the influence of a 10-d exposure to a total selected concentration of 98 μM nAl₂O₃ or to the equivalent molar concentration of ionic Al (AlCl₃) (196 μM) on the model plant Arabidopsis thaliana on the physiology (e.g., growth and photosynthesis, membrane damage) and the transcriptome using a high throughput state-of-the-art technology, RNA-seq. We found no evidence of nAl₂O₃ toxicity on photosynthesis, growth and lipid peroxidation. Rather the nAl₂O₃ treatment stimulated root weight and length by 48% and 39%, respectively as well as photosynthesis opening up the door to the use of nAl₂O₃ in biotechnology and nano agriculture. Transcriptomic analyses indicate that the beneficial effect of nAl₂O₃ was related to an increase in the transcription of several genes involved in root growth as well as in root nutrient uptake (e.g., up-regulation of the root hair-specific gene family and root development genes, POLARIS protein). By contrast, the ionic Al treatment decreased shoot and root weight of Arabidopsis thaliana by 57.01% and 45.15%, respectively. This toxic effect was coupled to a range of response at the gene transcription level including increase transcription of antioxidant-related genes and transcription of genes involved in plant defense response to pathogens. This work provides an integrated understanding at the molecular and physiological level of the effects of nAl₂O₃ and ionic Al in Arabidopsis.