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Complement component C3aR constitutes a novel regulator for chick eye morphogenesis.

Erika Grajales-Esquivel1, Agustin Luz-Madrigal2, Jeffrey Bierly1

  • 1Department of Biology, Miami University and Center for Visual Sciences at Miami University (CVSMU), Oxford, OH 45056, USA.

Developmental Biology
|June 4, 2017
PubMed
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This summary is machine-generated.

The complement component C3a receptor (C3aR) is crucial for chick eye development, regulating optic cup morphogenesis. Disrupting C3aR early in development causes severe eye defects, highlighting its essential role.

Area of Science:

  • Developmental Biology
  • Immunology
  • Ophthalmology

Background:

  • Complement components, including C3a and its receptor C3aR, are involved in various cellular functions.
  • Previous studies suggested C3a/C3aR signaling's role in chick retina regeneration.
  • The role of C3aR in embryonic eye development was previously unexplored.

Purpose of the Study:

  • To investigate the role of C3a/C3aR signaling in chick eye morphogenesis.
  • To determine the localization and function of C3aR during early eye development.

Main Methods:

  • Immunofluorescence staining to detect C3 and C3aR.
  • Loss-of-function studies using morpholinos and a blocking antibody against C3aR at different developmental stages.
  • Histological analysis to examine eye structure and cell populations.
Keywords:
C3C3aRColobomaComplementEye developmentMicrophthalmiaOptic vesicle

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  • Gene expression analysis for key developmental markers and signaling pathways.

    • Main Results:

    • C3 and C3aR are expressed during chick eye morphogenesis, with C3aR localized to the nucleus in the optic cup stage.
    • Early disruption of C3aR function leads to microphthalmia and coloboma, affecting optic stalk and optic cup formation.
    • Loss of C3aR function alters dorsal/ventral patterning, impacts Shh and Wnt signaling pathways, and increases cell death while decreasing proliferation.

    Conclusions:

    • C3aR is essential for proper chick optic cup morphogenesis.
    • C3aR acts as a critical regulator during early eye development, influencing cell fate, proliferation, and survival.
    • This study reveals a novel role for C3aR in embryonic eye development.