Related Experiment Video
Updated: Mar 1, 2026
10:21
Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
Published on: September 20, 2024
935
RNA-sequencing Identifies Novel Pathways in Sarcoidosis Monocytes
Jaya Talreja1, Pershang Farshi1, Adnan Alazizi2
1Department of Internal Medicine, Division of Pulmonary, Critical Care and Sleep Medicine, Wayne State University School of Medicine and Detroit Medical Center, Detroit, MI, 48201, USA.
Scientific Reports
|June 4, 2017
Summary
This study reveals significant gene expression differences in monocytes from sarcoidosis patients. Key pathways like phagocytosis and lysosome function were altered, offering new insights into sarcoidosis pathogenesis.
Area of Science:
- Immunology
- Genomics
- Molecular Biology
Background:
- Sarcoidosis is a complex systemic granulomatous disorder with unknown causes.
- Previous genetic studies have not fully elucidated its pathogenesis.
- Monocytes are implicated in sarcoidosis inflammation.
Purpose of the Study:
- To identify the gene expression profile in sarcoidosis monocytes.
- To determine altered cellular pathways in sarcoidosis monocytes using RNA-sequencing.
- To compare transcriptional signatures between sarcoidosis patients and healthy controls.
Main Methods:
- RNA-sequencing was performed on peripheral blood monocytes from sarcoidosis patients and healthy controls.
- Differential gene expression analysis was conducted to identify significant gene changes.
- Pathway enrichment analysis was used to understand the functional impact of altered gene expression.
Main Results:
- 2,446 differentially expressed genes were identified between sarcoidosis and control monocytes.
- Enriched pathways included ribosome, phagocytosis, lysosome, proteasome, oxidative phosphorylation, and metabolism.
- Phagocytosis and lysosomal pathway genes were upregulated, while proteasome and ribosomal pathway genes were downregulated in sarcoidosis monocytes.
Conclusions:
- Sarcoidosis monocytes exhibit distinct transcriptional profiles affecting key cellular functions.
- Altered pathways in phagocytosis, lysosome, proteasome, and ribosome function are implicated in sarcoidosis.
- Further research is needed to explore specific gene roles and interactions in sarcoidosis pathology.
More Related Videos
Related Concept Videos
01:20The JAK-STAT Signaling Pathway
13.4K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
13.4K
02:26NF-κB-dependent Signaling Pathway
10.1K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
10.1K
01:23Cystic Fibrosis: Pathogenesis
953
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
953
01:29Pharmacogenomics: Identification of New Drug Targets
53
Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...
53