Knockdown of long noncoding RNA GHET1 inhibits cell activation of gastric cancer
Hui Huang1, Wenjun Liao2, Xueqiang Zhu1
1Department of Cancer Center, Sichuan Provincial People's Hospital, Chengdu, Sichuian, 610000, China.
Objection:
The aim of this study was to evaluate the effects of lncRNA GHET1 in developing of Gastric Cancer.
Methods:
Collecting the 20 gastric cancer patients and evaluated the pathological of adjacent and tumor tissues by HE stating. We analyzed the protein expression of Numb in gastric cancer (GC) tissues by using IHC and the gene expression of Numb and GHET1 in adjacent and GC tissues by RT-PCR. The AGS cells were divided into 3 groups: Control (Co), NC and shRNA groups. Measuring the cell proliferation, apoptosis, cell cycle, invasion and migration abilities by MTT, flow cytometry, transwell and wound healing assays. We analyzed the relative signaling pathway by WB assay.
Results:
In the clinical analyzing, compared with adjacent tissues, the pathological was significantly changed in tumor tissues, the GHET1 gene and protein expressions were significantly increased in the GC tissues. In the cell experiment, down-regulation of GHET1 had suppressed the cell proliferation, invasion and migration activities and enhanced the cell apoptosis and G1 phase. We found that knockdown of GHET1 dramatically increased E-cadherin, while reducing fibronectin and vimentin.
Conclusion:
lncRNA GHET1 promoted AGS cells activations, and the results were shown that GHET1 dysregulation might be involved in the occurrence and development of gastric cancer. These results suggested that GHET1 might be a molecular marker for the progression of gastric cancer and a molecular target for targeted therapy.
Insights
Long non-coding RNA GHET1 is upregulated in gastric cancer tissues and promotes cancer progression. Downregulating GHET1 inhibits cell proliferation, invasion, and migration while enhancing apoptosis, suggesting it as a therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Gastric cancer (GC) is a significant global health concern.
- Long non-coding RNAs (lncRNAs) play crucial roles in various cancers.
- The specific role of lncRNA GHET1 in gastric cancer development requires further elucidation.
Purpose of the Study:
- To investigate the functional role of lncRNA GHET1 in gastric cancer.
- To evaluate GHET1 as a potential diagnostic marker and therapeutic target for GC.
Main Methods:
- Analysis of pathological and molecular differences between adjacent and tumor tissues from 20 GC patients.
- Assessment of GHET1 and Numb expression using HE staining, immunohistochemistry (IHC), and RT-PCR.
- In vitro studies using AGS cells to evaluate the impact of GHET1 knockdown on cell proliferation, apoptosis, cell cycle, invasion, and migration.
- Western blot assay to analyze relevant signaling pathways.
Main Results:
- Significant pathological changes and increased GHET1 gene/protein expression in GC tissues compared to adjacent tissues.
- Downregulation of GHET1 suppressed proliferation, invasion, and migration of AGS cells.
- GHET1 knockdown enhanced apoptosis and promoted G1 phase cell cycle arrest.
- Knockdown of GHET1 increased E-cadherin expression and decreased fibronectin and vimentin levels.
Conclusions:
- lncRNA GHET1 promotes AGS cell activation and is implicated in gastric cancer occurrence and development.
- GHET1 may serve as a valuable molecular marker for gastric cancer progression.
- GHET1 represents a potential molecular target for gastric cancer-specific therapies.
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