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Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

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Related Experiment Video

Updated: Mar 1, 2026

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
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Azithromycin Modulates 3',5'-cyclic Diguanylic Acid Signaling in Pseudomonas aeruginosa.

Soichiro Kimura1, Nobuaki Mori2, Toshihiro Kai3

  • 1Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, Japan.

Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy
|June 6, 2017
PubMed
Summary

Azithromycin, a macrolide antibiotic, impacts the 3

Keywords:
MacrolidePseudomonas aeruginosac-di-GMP

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Pharmacology

Background:

  • Macrolides modulate host immunity and bacterial virulence.
  • The 3',5'-cyclic diguanylic acid (c-di-GMP) signaling pathway regulates Pseudomonas aeruginosa virulence.

Purpose of the Study:

  • To investigate the effect of azithromycin on the c-di-GMP signaling system in Pseudomonas aeruginosa.
  • To identify specific genes regulated by azithromycin within this pathway.

Main Methods:

  • DNA microarray analysis to identify azithromycin-affected genes.
  • Gene expression analysis (time- and dose-dependent).
  • Construction of a PA2567 deletion mutant and evaluation in a mouse pneumonia model.

Main Results:

  • Azithromycin significantly repressed the expression of gene PA2567, involved in c-di-GMP metabolism.
  • Deletion of PA2567 impaired virulence factors and quorum sensing.
  • PA2567 deletion led to significantly reduced bacterial load in a mouse pneumonia model.

Conclusions:

  • Azithromycin modulates the intracellular c-di-GMP signaling pathway in Pseudomonas aeruginosa.
  • The macrolide azithromycin influences bacterial virulence through regulation of c-di-GMP metabolism.