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MC3T3 osteoblast-like cells cultured at alkaline pH: Microarray data (Affymetrix GeneChip Mouse 2.0 ST)
Anne-Marie Galow1, Alexander Rebl2, Dirk Koczan3
1University of Rostock, Gertrudenstr. 11a, 18057 Rostock, Germany.
Data in Brief
|June 6, 2017
Summary
Alkaline pH significantly alters gene expression in osteoblasts, impacting bone remodeling. Pathway analysis revealed distinct cellular responses and highlighted the central role of p38 MAP kinase in pH-dependent regulation.
Area of Science:
- Biochemistry
- Molecular Biology
- Cell Biology
Background:
- Bone remodeling is critically regulated by pH.
- Comprehensive gene expression data for alkaline pH effects on osteoblasts are lacking.
Purpose of the Study:
- To investigate the impact of alkaline pH on osteoblast gene expression.
- To identify differentially expressed genes and associated pathways in osteoblasts cultured at varying pH levels.
Main Methods:
- MC3T3-E1 osteoblast-like cells were cultured at pH 7.4, 7.8, and 8.4 for 14 days.
- Microarray analysis (Affymetrix GeneChips) identified differential gene expression.
- Real-time PCR validated expression of key genes (DMP1, FABP4, SFRP2, TNFRSF19, ATF4).
- Pathway and functional gene network analyses were performed.
Main Results:
- Alkaline pH induced significant changes in osteoblast gene expression.
- Datasets from pH 7.8 vs. 7.4 and pH 8.4 vs. 7.4 shared many differentially expressed genes.
- Comparing pH 8.4 vs. 7.8 revealed distinct pathways, not just amplified effects.
- Three distinct pH-dependent cellular response networks were identified, with p38 MAP kinase playing a central role.
Conclusions:
- Alkaline pH profoundly influences osteoblast gene expression and cellular responses.
- Distinct pathways are activated at higher alkaline pH levels.
- p38 MAP kinase is a key regulator in pH-mediated osteoblast function.
