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A quantitative assay for Amaranthus palmeri identification.

Brent P Murphy1, Diane E Plewa2, Elizabeth Phillippi2

  • 1Department of Crop Sciences, University of Illinois, Urbana, IL, USA.

Pest Management Science
|June 6, 2017
PubMed
Summary

A new quantitative polymerase chain reaction (qPCR) assay can accurately detect Palmer amaranth (Amaranthus palmeri) in plant and seed samples. This rapid screening method helps prevent the spread of this invasive weed species.

Keywords:
Amaranthus palmeriConservation Reserve ProgramqPCRspecies identification

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Area of Science:

  • Agronomy
  • Botany
  • Molecular Biology

Background:

  • Amaranthus palmeri, an invasive weed, has been introduced to the Midwestern USA via Conservation Reserve Program seeding mixes.
  • Effective management strategies require rapid methods for identifying and monitoring this species' spread.

Purpose of the Study:

  • To develop and validate a molecular assay for the specific detection of Amaranthus palmeri.
  • To provide a tool for rapid species screening to mitigate the ecological and agricultural risks associated with Amaranthus palmeri.

Main Methods:

  • Development of species-specific nucleotide polymorphism markers in the ribosomal DNA internal transcribed spacer region.
  • Design and validation of a quantitative polymerase chain reaction (qPCR) assay for Amaranthus palmeri detection.

Main Results:

  • The developed qPCR assay accurately identified Amaranthus palmeri in various sample types, including single plants, mixed-plant samples, and seed mixtures.
  • The assay demonstrated high specificity, successfully distinguishing Amaranthus palmeri from 12 other Amaranthus species.
  • Sensitivity analysis confirmed the assay's ability to detect a single Amaranthus palmeri seed in a pool of 100 Amaranthus spp. seeds.

Conclusions:

  • A validated qPCR assay provides a reliable method for distinguishing Amaranthus palmeri from related Amaranthus species.
  • This molecular tool facilitates rapid and accurate identification, crucial for managing the invasive spread of Amaranthus palmeri.