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Updated: Mar 1, 2026

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
Published on: April 18, 2016
Detection of Coxiella burnetii in Urban River Water
Emilio D'Ugo1, Massimo Sdanganelli1, Cinzia Grasso1
11 Quality and Fishfarm Unit, Department of Environmental and Primary Prevention, Istituto Superiore di Sanità , Rome, Italy .
Abstract:
Previous molecular-based studies have identified microorganisms of zoonotic and human nature in surface waters. Contaminated water can lead to human health issues, and the detection of pathogenic microorganisms is a valuable tool for the prevention of their spread. Water samples were taken from the River Tiber in and out of the city of Rome. Genetic analysis of the sequences obtained showed the presence of Coxiella burnetii in both the analyzed sites. Blast analysis showed that two sequences were identical to each other. Sequences from the polluted site showed high homology with different strains of C. burnetii. In this article, we report for the first time the presence of C. burnetii in environmental waters.
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