New applications of genetically modified Pseudomonas aeruginosa for toxicity detection in water
Dengbin Yu1, Yang-Chun Yong2, Changyu Liu1
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, 5625 Renmin Street, Changchun 130022, Jilin Province, PR China.
Abstract:
A novel mediator-free method based on genetically modified bacteria was developed for detecting water toxicity, where genetically modified Pseudomonas aeruginosa (GM P. aeruginosa) was selected as the biosensor strain and pyocyanin (PYO) produced by this strain was used as the indicator. The toxicity response of GM P. aeruginosa to 3, 5-dichlorophenol (3, 5-DCP) was measured electrochemically and spectroscopically, and the half maximal inhibitory concentration (IC50) of 3, 5-DCP was determined to be 15.1 mg/L. Strikingly, the toxicity of sample solution with 3, 5-DCP could also be estimated visually by naked eyes at a concentration as low as 10 mg/L. The present study provided a convenient, sensitive and cost-effective method for water toxicity detection, and extended biosensing application of the genetically modified bacterium.
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