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Simultaneous Subtotal Pancreatectomy and Streptozotocin Injection for Diabetes Modeling in Cynomolgus Monkeys
1Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Transplantation Proceedings
|June 7, 2017
Summary
A novel method using subtotal pancreatectomy and low-dose streptozotocin (STZ) effectively induces insulin-dependent diabetes mellitus (IDDM) in nonhuman primates. This approach also yields sufficient islets for transplantation, avoiding harm to additional animals.
Area of Science:
- Endocrinology
- Surgical Research
- Transplantation Biology
Background:
- Stable induction of insulin-dependent diabetes mellitus (IDDM) and islet isolation are crucial for islet transplantation research in nonhuman primates.
- Current methods like total pancreatectomy for IDDM induction and islet procurement cause significant exocrine dysfunction and associated morbidities.
Purpose of the Study:
- To develop a safer and more effective method for inducing IDDM and procuring islets in nonhuman primates for transplantation studies.
- To overcome the limitations of streptozotocin (STZ) toxicity and the need for additional animals in islet isolation.
Main Methods:
- A combined approach of subtotal pancreatectomy and low-dose streptozotocin (STZ) injection (60 mg/kg) was employed in twelve cynomolgus monkeys.
- This procedure aimed to induce IDDM while simultaneously obtaining pancreatic tissue for islet isolation.
Main Results:
- All twelve monkeys recovered from the procedure without complications, and successful induction of IDDM was confirmed.
- An average of 57,691 ± 16,050 islets were successfully isolated from the resected pancreas.
- These isolated islets were suitable for subsequent allotransplantation into other recipients.
Conclusions:
- Simultaneous subtotal pancreatectomy and low-dose STZ injection provide an effective and safe strategy for creating an IDDM nonhuman primate model.
- This refined technique yields adequate fresh islet cells for allotransplantation, eliminating the need to sacrifice additional animals for islet procurement.
