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Evaluation of Cassia tora Linn. against Oxidative Stress-induced DNA and Cell Membrane Damage
R Sunil Kumar1, Ramesh Balenahalli Narasingappa1, Chandrashekar G Joshi2
1Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Hassan, Bengaluru, Karnataka, India.
Objective:
The present study aims to evaluate antioxidants and protective role of Cassia tora Linn. against oxidative stress-induced DNA and cell membrane damage.
Materials And Methods:
The total and profiles of flavonoids were identified and quantified through reversed-phase high-performance liquid chromatography. In vitro antioxidant activity was determined using standard antioxidant assays. The protective role of C. tora extracts against oxidative stress-induced DNA and cell membrane damage was examined by electrophoretic and scanning electron microscopic studies, respectively.
Results:
The total flavonoid content of CtEA was 106.8 ± 2.8 mg/g d.w.QE, CtME was 72.4 ± 1.12 mg/g d.w.QE, and CtWE was 30.4 ± 0.8 mg/g d.w.QE. The concentration of flavonoids present in CtEA in decreasing order: quercetin >kaempferol >epicatechin; in CtME: quercetin >rutin >kaempferol; whereas, in CtWE: quercetin >rutin >kaempferol. The CtEA inhibited free radical-induced red blood cell hemolysis and cell membrane morphology better than CtME as confirmed by a scanning electron micrograph. CtEA also showed better protection than CtME and CtWE against free radical-induced DNA damage as confirmed by electrophoresis.
Conclusion:
C. tora contains flavonoids and inhibits oxidative stress and can be used for many health benefits and pharmacotherapy.
