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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Preliminary Study on Determining the Sequence of Intersecting Lines by Fluorescence Technique.

Biao Li¹, Guo Liang Ouyang¹, Peng Nan Zhao¹

¹Department of Document Examination, National Police University of China, No.83 Tawan Str., 110854, Shenyang, China.

Journal of Forensic Sciences

|June 7, 2017

Summary

A fluorescence technique successfully determines the sequence of intersecting lines from various writing instruments and printers. This method aids in questioned document examinations, though it struggles with blended lines.

Keywords:

fluorescence technique forensic science ink intersecting lines questioned documents sequence of intersecting lines

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Area of Science:

Forensic Science
Document Examination

Background:

Determining the sequence of intersecting lines is crucial in forensic document analysis.
Previous methods may have limitations in analyzing diverse ink and printing technologies.

Purpose of the Study:

To evaluate the efficacy of a fluorescence technique for sequencing intersecting lines from various sources.
To assess the applicability of this method across different ink types and printing methods.

Main Methods:

Utilized a SteREO Discovery.V20 microscope with ZEN Blue Lite software.
Applied a fluorescence technique to analyze intersecting lines from ballpoint pens, gel pens, fountain pens, stamp-pad inks, carbon paper, photocopiers, laser printers, and inkjet printers.

Main Results:

The fluorescence technique proved highly successful for sequencing intersecting lines from ballpoint pens, gel pens, fountain pens, photocopiers, laser printers, inkjet printers, and inkpad inks.
The method also showed success with ballpoint pen, photocopier, laser printer, and stamp-pad inks under most conditions.
The technique was unsuccessful when analyzing intersecting lines that were blended together.

Conclusions:

Fluorescence microscopy is a valuable tool for determining the sequence of intersecting lines in questioned document examinations.
The technique demonstrates broad applicability across a wide range of common writing and printing materials.
Limitations exist for blended ink lines, requiring further investigation or alternative methods.