Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Preparation of Amides

Preparation of Amides

Amides are synthesized by treating carboxylic acids with amines in the presence of dehydrating agents like dicyclohexylcarbodiimide (DCC).
The DCC-promoted synthesis of amides begins with the protonation of DCC by carboxylic acid. The protonation makes it a better acceptor. Next, the addition of carboxylate to the protonated carbodiimide gives a reactive acylating agent.
Subsequently, the amine acts as a nucleophile that attacks the acylating agent to form a tetrahedral intermediate. In the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Probing the <i>C</i> <sub>3</sub> symmetry of gramicidin S.

RSC chemical biology·2026

Same author

Inhibition of PfCLK3 interferes with malaria parasite RNA splicing and explains the mechanism of action of a new class of antimalarial candidates.

Antimicrobial agents and chemotherapy·2026

Same author

On-Resin Synthesis and Diversification of Macrocyclic Disulfide Bridge Peptidomimetics via Bifunctional 5-Iodo-1,4-Triazoles.

The Journal of organic chemistry·2026

Same author

Use of Human Dose Prediction Metrics to Enable Discovery of AZD3470, an MTA-Cooperative PRMT5 Inhibitor in Clinical Evaluation.

Journal of medicinal chemistry·2026

Same author

Discovery, Structure-Activity Relationship, and Functional Characterization of a Chromenopyrrole Series as Orthosteric Antagonists of GPR84.

Journal of medicinal chemistry·2026

Same author

Selective small molecule targeting of KDM4 as a therapeutic strategy to reduce proliferation of acute myeloid leukaemia.

British journal of haematology·2026

Search research articles

Related Experiment Video

Updated: Mar 1, 2026

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

Solid-Phase Synthesis of Oxetane Modified Peptides.

Jonathan D Beadle¹, Astrid Knuhtsen², Alex Hoose²

¹Department of Chemistry, University of Warwick , Gibbet Hill Road, Coventry CV4 7AL, U.K.

Organic Letters

|June 7, 2017

Summary

Researchers developed a novel method using solid-phase peptide synthesis (SPPS) to create peptidomimetics with oxetane rings. This technique allows for the incorporation of modified building blocks into peptide chains, yielding high-purity peptide derivatives.

More Related Videos

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

Related Experiment Videos

Last Updated: Mar 1, 2026

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

Area of Science:

Medicinal Chemistry
Organic Synthesis
Peptide Chemistry

Background:

Solid-phase peptide synthesis (SPPS) is a cornerstone for creating peptide-based therapeutics and research tools.
Incorporating non-natural modifications into peptide backbones can enhance stability and alter biological activity.
Developing efficient methods for synthesizing modified peptides is crucial for advancing drug discovery.

Purpose of the Study:

To develop a novel method for synthesizing peptidomimetics containing oxetane rings within the peptide backbone.
To demonstrate the utility of oxetane-containing dipeptide building blocks in conventional Fmoc SPPS.
To produce high-purity backbone-modified peptide derivatives for potential therapeutic applications.

Main Methods:

Synthesis of oxetane-containing dipeptide building blocks in solution through a three-step process.
Integration of these modified building blocks into peptide chains using standard Fmoc SPPS.
Purification and characterization of the resulting backbone-modified peptides.

Main Results:

Successfully synthesized oxetane-containing dipeptide building blocks.
Demonstrated efficient incorporation of these building blocks into growing peptide chains via Fmoc SPPS.
Produced a range of high-purity peptidomimetics, including modified bradykinin, Met-enkephalin, and Leu-enkephalin.

Conclusions:

The described methodology provides a robust route for incorporating oxetane rings into peptide backbones.
This approach enables the synthesis of novel peptidomimetics with potential for improved pharmacological properties.
The developed technique is versatile and applicable to various peptide sequences.