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Related Experiment Videos

Beta adrenergic receptors in human cavernous tissue.

C B Dhabuwala, C V Ramakrishna, G F Anderson

    The Journal of Urology
    |April 1, 1985
    PubMed
    Summary

    Human cavernous tissues possess beta-2 adrenergic receptors, with stable binding affinities in postmortem samples. Estrogen therapy in transsexual patients did not alter beta adrenergic receptor density in these tissues.

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    Urology·2003

    Area of Science:

    • Pharmacology
    • Urology
    • Endocrinology

    Background:

    • Beta adrenergic receptors play a role in smooth muscle function.
    • Human cavernous tissue is a relevant model for studying adrenergic receptor pharmacology.

    Purpose of the Study:

    • To characterize beta adrenergic receptors in human cavernous tissue.
    • To assess the stability of these receptors in postmortem tissue.
    • To investigate the effect of estrogen therapy on receptor density.

    Main Methods:

    • Radioligand binding assays using 125I-iodocyanopindolol.
    • Isotherm binding studies and displacement assays.
    • Scatfit non-linear regression analysis for receptor characterization.

    Main Results:

    • Normal cavernous tissue exhibited a receptor density of 9.1 fmoles/mg with a KD of 23 pM.
    • Postmortem tissue showed stable receptor density and affinity, indicating reliability for research.
    • Transsexual patients receiving estrogen therapy did not display altered beta adrenergic receptor density compared to controls.
    • Displacement studies indicated the presence of beta-2 adrenergic receptors, with 1-propranolol showing the highest affinity.

    Conclusions:

    • Human cavernous tissue expresses beta-2 adrenergic receptors.
    • Postmortem tissue is suitable for receptor binding studies.
    • Estrogen therapy does not significantly impact beta adrenergic receptor density in this tissue.

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