Microbial decolorization and detoxification of emerging environmental pollutant: Cosmetic hair dyes
Swati Maiti1, Sudarson Sekhar Sinha2, Mukesh Singh1
1Department of Biotechnology, Haldia Institute of Technology, Haldia, Purba, Medinipur 721657, West Bengal, India.
Abstract:
Since the usage of hair dyes has increased in recent time, the removal of residual dye from environment is also an emerging issue. Hair dye contains mixture of chemicals including genotoxic chemical, p-phenylenediamine (p-PD or PPD). The present study reports bioremediation of hair dye using bacteria isolated from saloon effluent. Sugarcane bagasse powder (SBP) was used as a source of nutrient and surface for bacterial growth. The 16S rDNA sequencing confirmed the isolate as Enterobacter cloacae which was designated as DDB I. The decolourization of dye was studied using UV-vis spectrophotometer. The detoxification study was conducted on microbes isolated from fresh ponds using well diffusion assay. The 1mg/ml of dye was effectively decolourised within 18h of DDB I treatment in the minimal medium containing 30mg/ml of SBP.
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