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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

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Microarray expression profiling in the denervated hippocampus identifies long noncoding RNAs functionally involved in

Bingying Deng¹, Xiang Cheng¹, Haoming Li¹

¹Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.

BMC Molecular Biology

|June 8, 2017

Summary

This summary is machine-generated.

This study identifies novel long noncoding RNAs (lncRNAs) involved in hippocampal neurogenesis. A newly discovered lncRNA, lncRNA2393, promotes neural stem cell proliferation in the denervated hippocampus.

Keywords:

Hippocampus Long noncoding RNA Microarray Neural stem cells Neurogenesis

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Published on: April 12, 2015

Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

The denervated hippocampus creates a supportive environment for neural progenitor survival and differentiation.
While numerous long noncoding RNAs (lncRNAs) have been identified, few are known to regulate hippocampal neurogenesis.

Purpose of the Study:

To identify lncRNAs involved in hippocampal neurogenesis using microarray expression profiling.
To investigate the potential roles of these identified lncRNAs in hippocampal neurogenesis.

Main Methods:

Microarray expression profiling to compare lncRNA expression in denervated versus normal hippocampi.
Fluorescence in situ hybridization (FISH) to determine the localization of lncRNA2393.
In vitro experiments to assess the effect of lncRNA2393 knockdown on neural stem cells (NSCs).

Main Results:

Microarray analysis revealed 74 activated and 29 repressed lncRNAs in the denervated hippocampus.
A novel lncRNA, lncRNA2393, was identified as a neural regulator, with its expression activated in the denervated hippocampus.
lncRNA2393 is localized to the subgranular zone of the dentate gyrus and the cytoplasm of NSCs, and its knockdown impairs NSC proliferation.

Conclusions:

Altered lncRNA expression in the denervated hippocampus microenvironment promotes neurogenesis.
The identified lncRNA2393, found in hippocampal neural stem cells, promotes NSC proliferation in vitro.
Further research is needed to elucidate the molecular mechanisms driving lncRNA2393 expression and its clinical applications for central nervous system disorders.