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Related Experiment Video

Updated: Feb 4, 2026

Basophil Activation Test for Allergy Diagnosis

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

For Certain, SIRT4 Activities!

Surinder Kumar¹, David B Lombard²

¹Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA.

Trends in Biochemical Sciences

|June 8, 2017

Summary

This summary is machine-generated.

The sirtuin 4 (SIRT4) protein

Keywords:

HMGylation MGcylation MGylation SIRT4 Sirtuins

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Last Updated: Feb 4, 2026

Basophil Activation Test for Allergy Diagnosis

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Published on: May 31, 2021

Analyzing Neural Activity and Connectivity Using Intracranial EEG Data with SPM Software

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Measurement of Larval Activity in the Drosophila Activity Monitor

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Published on: April 30, 2015

Area of Science:

Biochemistry
Molecular Biology
Metabolic Regulation

Background:

Sirtuin 4 (SIRT4) is a key metabolic regulator, but its precise enzymatic function remains unclear.
Understanding SIRT4's activity is crucial for deciphering metabolic pathways and disease mechanisms.

Purpose of the Study:

To elucidate the enzymatic activity of SIRT4.
To identify the specific substrates and biological relevance of SIRT4's deacylase function.

Main Methods:

Biochemical assays to test deacylase activity.
Mass spectrometry to identify modified substrates.
Analysis of leucine catabolism pathways.

Main Results:

SIRT4 exhibits deacylase activity against novel lysine modifications.
These modifications arise from reactive acyl-CoAs produced during leucine catabolism.
Identified specific acyl-CoA derivatives as substrates for SIRT4.

Conclusions:

SIRT4 functions as a deacylase targeting specific lysine modifications.
This activity links leucine metabolism to protein post-translational modifications.
Uncovers a novel regulatory mechanism in cellular metabolism.