Gene Expression Analysis in Rat Pancreas Observed with Whole-Transcript Exon Array after Ventromedial Hypothalamic
1Division of Modern Medical Technology, Institute for Clinical Research, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Kure, Japan.
Annals of Neurosciences
|June 8, 2017
Summary
Ventromedial hypothalamic (VMH) lesions impact gene expression in the rat pancreas. Whole-transcript arrays reveal differences in probe identification compared to 3' based arrays, affecting cellular mechanism studies.
Area of Science:
- Molecular Biology
- Genomics
- Neuroscience
Background:
- Previous studies using 3' based arrays (Affymetrix Rat Genome 230 2.0) showed VMH lesions affect cell proliferation, neuron, and metabolism genes.
- The newer Rat Gene 1.0 ST array interrogates entire mRNA transcripts and uses DNA targets, differing from older 3' based arrays.
Purpose of the Study:
- To assess the impact of new array technology (whole-transcript exon arrays) on gene expression analysis performance.
- To evaluate differences in probe identification between 3' based and whole-transcript arrays.
Main Methods:
- Utilized the Rat Gene 1.0 ST array, a whole-transcript rat exon array.
- Examined cellular mechanisms of gene regulation in rat pancreas following VMH lesions.
Main Results:
- VMH lesions were found to regulate genes involved in enzymes, metabolism, transport, binding, differentiation, migration, morphology, apoptosis, neurons, and immunity.
- Significant differences were observed in the probes identified by the Rat Gene 1.0 ST array compared to the Rat Genome 230 2.0 array.
Conclusions:
- Confirms that VMH lesions can affect the expression of numerous functional genes within the rat pancreas.
- Highlights the importance of array technology differences in interpreting gene expression data related to VMH lesions.
