Gene Expression Analysis in Rat Pancreas Observed with Whole-Transcript Exon Array after Ventromedial Hypothalamic
1Division of Modern Medical Technology, Institute for Clinical Research, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Kure, Japan.
Background:
It was reported previously that using Affymetrix Rat Genome 230 2.0, one of a class of standard 3' based arrays, ventromedial hypothalamic (VMH) lesions affected the expressions of cell proliferation-related genes, neuron-related genes, and metabolism-related genes. The released Affymetrix Rat Gene 1.0 ST array has 2 major differences compared with standard 3' based arrays, including Rat Genome 230 2.0: it interrogates the entire mRNA transcript and uses DNA targets.
Purpose:
This study is aimed at assessing the impact of these differences on the array performance.
Methods:
The study used Rat Gene 1.0 ST array, one of a class of whole-transcript rat exon arrays to examine the cellular mechanisms of gene regulation in the rat pancreas after VMH lesions.
Results:
Although the results showed that VMH lesions regulated genes involved in enzymes, metabolism, transport, binding differentiation, migration, morphology, apoptosis, neuron and immunity, the probes identified by these 2 arrays were remarkably different.
Conclusion:
This study also confirmed that VMH lesions may affect the expression of many functional genes in rat pancreas.
