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Updated: Mar 1, 2026
In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy
Published on: January 25, 2008
Tracking Plasma Cell Differentiation in Living Mice with Two-Photon Microscopy
Carolin Ulbricht1, Randall L Lindquist2, Laura Tech2
1Immune Dynamics and Intravital Microscopy, Charité Universitätsmedizin, Charitéplatz 1, 10117, Berlin, Germany.
Due to the multitude of cell types involved in the differentiation of plasma cells during the germinal center reaction, and due to a lack of in vitro systems, which recapitulate germinal centers, the most suitable way to study plasma cell generation in germinal centers is in vivo. In this chapter we describe how to induce humoral immune responses to defined model antigens and how to visualize and track plasma cells and their interactions with other cells in the lymph nodes of living mice.
Due to the multitude of cell types involved in the differentiation of plasma cells during the germinal center reaction, and due to a lack of in vitro systems, which recapitulate germinal centers, the most suitable way to study plasma cell generation in germinal centers is in vivo. In this chapter we describe how to induce humoral immune responses to defined model antigens and how to visualize and track plasma cells and their interactions with other cells in the lymph nodes of living mice.
