An international survey about nail histology processing techniques.
Christina Wlodek1, Pauline Lecerf2, Josette Andre3
1Dermatology Department, Bristol Royal Infirmary, Bristol, UK.
Journal of Cutaneous Pathology
|June 8, 2017
Summary
Nail histopathology techniques vary internationally, with most labs using formalin fixation and paraffin embedding. Standard processing shows minor differences from normal skin procedures.
Area of Science:
- Dermatopathology
- Histotechnology
- Nail Diseases
Background:
- Limited data exists on current nail histopathology techniques.
- International variations in nail histopathology practices are not well-documented.
Purpose of the Study:
- To investigate and document the nail histopathology techniques employed globally.
- To identify common practices and variations in nail sample processing.
Main Methods:
- An international online survey was distributed to members of the European Nail Society and Council for Nail Disorders.
- Data was collected on specimen fixation, embedding, nail softening, sectioning, and staining methods.
Main Results:
- Fifty-seven respondents from twenty countries participated, including dermatologists, podiatrists, and pathologists.
- Paraffin embedding was most common (90%), with formalin fixation (6-48 hours) and nail softening (71%) also prevalent.
- Hematoxylin and eosin (H&E) and Periodic acid Schiff (PAS) were routine stains, with Fontana-Masson favored for melanin/hemoglobin and Melan-A for pigmented lesions.
Conclusions:
- Nail histopathology processing exhibits minor deviations compared to standard skin processing.
- Standardization of nail histopathology techniques could improve diagnostic consistency.
