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Updated: Mar 1, 2026

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
Published on: January 27, 2012
Monitoring focal adhesion kinase phosphorylation dynamics in live cells
Nur P Damayanti1, Kevin Buno2, Nagarajan Narayanan3
1Bindley Bioscience Center, Purdue Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, USA. josephi@purdue.edu and Indiana University School of Medicine, 980 West Walnut St. R3 C312, Indianapolis, Indiana 46202, USA.
Abstract:
Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.
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