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[Dipeptidyl peptidase IV. Purification for use in peptide sequencing]
Biological Chemistry Hoppe-Seyler
|February 1, 1985
Summary
Researchers purified dipeptidyl peptidase IV from pig kidney. This highly pure enzyme is suitable for peptide-sequence analysis.
Area of Science:
- Biochemistry
- Enzymology
Context:
- Dipeptidyl peptidase IV (DPP IV) is a key enzyme involved in various physiological processes.
- Efficient isolation and purification methods are crucial for studying enzyme function and application.
Purpose:
- To isolate and purify dipeptidyl peptidase IV (DPP IV) from porcine kidney.
- To characterize the purity and specific activity of the purified enzyme.
Summary:
- Dipeptidyl peptidase IV was successfully isolated and purified from pig kidney using chromatography on Gly-Pro-AH-Sepharose.
- The purified enzyme exhibited a specific activity of 24.9 U/mg protein.
- The preparation was found to be free from contaminating aminopeptidase, dipeptidase, and prolyl endopeptidase activities.
Impact:
- Provides a highly purified DPP IV enzyme preparation.
- The enzyme's purity and lack of other protease activities make it suitable for peptide-sequence analysis.
- Facilitates further research into DPP IV's role in biological systems and its potential therapeutic applications.