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Related Experiment Video

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Novel approaches in function-driven single-cell genomics.

Devin F R Doud1, Tanja Woyke1

  • 1DOE Joint Genome Institute, Walnut Creek, CA 94598, USA.

FEMS Microbiology Reviews
|June 8, 2017
PubMed
Summary
This summary is machine-generated.

Function-driven single-cell genomics targets specific microbial cells for genome sequencing. This approach reveals microbial functions and activities in complex environments, advancing our understanding of microbial communities.

Keywords:
function-driven sequencingmicrobial dark mattersingle-cell activitysingle-cell genomics

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Area of Science:

  • Microbiology
  • Genomics
  • Environmental Science

Background:

  • Advances in sequencing and bioinformatics illuminate microbial communities.
  • Marker genes identify 'who is there,' but 'what they are doing' remains challenging.
  • Understanding microbial roles in biogeochemistry is crucial.

Purpose of the Study:

  • To review strategies for function-driven single-cell genomics.
  • To highlight its role in understanding microbial functions.
  • To outline future research directions.

Main Methods:

  • Function-based screening to isolate single cells.
  • Targeted genome sequencing of selected single cells.
  • Subsampling complex microbial communities.

Main Results:

  • Enables definitive linking of genomes to functions.
  • Improves genome annotation and reveals in-situ activity.
  • Facilitates analysis of uncharacterized gene products.

Conclusions:

  • Function-driven single-cell genomics is a powerful approach.
  • It advances the understanding of microbial community functioning.
  • It opens new avenues for exploring microbial dark matter.