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pH-dependent lipid vesicle interactions with plasma polymerized thin films.

Hannah J Askew1, Mirren Charnley2, Karyn L Jarvis3

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Plasma polymers control lipid vesicle adsorption and supported lipid bilayer formation. Surface chemistry and pH dictate interactions, enabling patterned lipid structures for applications like biosensing and drug delivery.

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Area of Science:

  • Surface science
  • Materials science
  • Biophysics

Background:

  • Model lipid vesicle and supported lipid bilayer (SLB) systems are crucial for biosensing, cell membrane mimics, and drug delivery.
  • Vesicle adsorption and collapse onto surfaces is a key method for creating SLBs, but surface property effects are poorly understood.
  • Plasma polymers offer tunable surface properties for various materials.

Purpose of the Study:

  • To investigate lipid vesicle interactions with plasma polymerized acrylic acid (ppAAc) and allylamine (ppAAm) films.
  • To explore how surface chemistry and pH influence vesicle adsorption, collapse, and supported lipid bilayer (SLB) formation.
  • To assess the fluidity of lipid structures formed on patterned plasma polymer surfaces.

Main Methods:

  • Quartz crystal microbalance with dissipation (QCM-D) to monitor vesicle adsorption and stability.
  • Fluorescence recovery after photobleaching (FRAP) to measure lipid fluidity in formed bilayers.
  • Utilizing ppAAc, ppAAm, and ppAAc/ppAAm micropatterned surfaces under varying pH conditions.

Main Results:

  • Vesicle interactions strongly depended on plasma polymer chemistry and buffer pH.
  • Stable vesicle adsorption occurred on ppAAm across a wide pH range, while ppAAc showed pH-dependent interactions.
  • pH-induced vesicle collapse and SLB formation were observed, with patterned surfaces allowing distinct fluid and immobile lipid regions.

Conclusions:

  • Plasma polymer surface chemistry and pH are critical parameters for controlling lipid vesicle adsorption and SLB formation.
  • Patterned plasma polymer films enable spatially controlled creation of lipid structures with tunable fluidity.
  • This approach offers a versatile platform for fabricating functional lipid interfaces on diverse substrates for advanced applications.