Related Experiment Video

Updated: Mar 1, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.4K

Phosphorylated 4E-BP1 is associated with tumor progression and adverse prognosis in colorectal cancer

Y Chen, J Wang, H Fan

    Neoplasma
    |June 9, 2017
    PubMed

    Insights

    High levels of phosphorylated 4E-BP1 (p-4E-BP1) are linked to colorectal cancer progression. This protein may serve as a novel biomarker for predicting patient outcomes and survival in colorectal cancer (CRC).

    Area of Science:

    • Oncology
    • Molecular Biology
    • Biochemistry

    Background:

    • Phosphorylation of eukaryotic translation initiation factor 4E (eIF4E)-binding protein (4E-BP1) releases eIF4E, promoting cancer-associated protein synthesis.
    • Elevated p-4E-BP1 levels are implicated in various cancers, but its role in colorectal cancer (CRC) requires further elucidation.

    Purpose of the Study:

    • To evaluate the expression of phosphorylated 4E-BP1 (p-4E-BP1) in colorectal cancer (CRC) tissues.
    • To investigate the correlation between p-4E-BP1 levels and clinicopathological factors in CRC patients.
    • To assess the prognostic value of p-4E-BP1 for patient survival in CRC.

    Main Methods:

    • Immunohistochemistry and Western blot were employed to detect p-4E-BP1 levels in 164 CRC patients.
    • mRNA and protein expression of 4E-BP1 were compared between tumor and adjacent normal colorectal tissues.
    • Cox regression analysis was utilized to determine the prognostic significance of p-4E-BP1.

    Main Results:

    • A high level of p-4E-BP1 was observed in 57.9% of CRC patients.
    • p-4E-BP1 levels significantly correlated with tumor differentiation, invasive depth, lymph node metastasis, and TNM stage.
    • While 4E-BP1 mRNA and protein levels did not differ, p-4E-BP1 was markedly increased in CRC tissues.

    Conclusions:

    • Elevated p-4E-BP1 is associated with tumor progression and adverse prognosis in colorectal cancer.
    • p-4E-BP1 serves as an independent adverse prognostic factor for overall survival and progression-free survival in CRC.
    • p-4E-BP1 shows potential as a novel biomarker for predicting clinical outcomes in colorectal cancer patients.
    Keywords:
    biomarkercolorectal cancerp-4E-BP1tumor progression prognosis.

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